Publication: Generation of three human induced pluripotent stem cell lines with IRX5 knockout and knockin genetic editions using CRISPR-Cas9 system
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Canac, Robin
Caillaud, Amandine
Cimarosti, Bastien
Girardeau, Aurore
Hamamy, Hanan
Bonnard, Carine
Al Sayed, Zeina R.
David, Laurent
Poschmann, Jeremie
Lemarchand, Patricia
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Studies on animal models have shown that Irx5 is an important regulator of cardiac development and that it regulates ventricular electrical repolarization gradient in the adult heart. Mutations in IRX5 have also been linked in humans to cardiac conduction defects. In order to fully characterize the role of IRX5 during cardiac development and in cardiomyocyte function, we generated three genetically-modified human induced pluripotent stem cell lines: two knockout lines (heterozygous and homozygous) and a knockin HA-tagged line (homozygous).
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Elsevier
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Stem Cell Research
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DOI
10.1016/j.scr.2021.102627