Publication: Cloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase from Mus musculus and Homo sapiens
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Singh, Nisha
Halliday, Amy C.
Knight, Matthew
Lowe, Edward
Churchill, Grant C.
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NO
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Abstract
Inositol monophosphatase (IMPase) catalyses the hydrolysis of inositol monophosphate to inositol and is crucial in the phosphatidylinositol (PI) signalling pathway. Lithium, which is the drug of choice for bipolar disorder, inhibits IMPase at therapeutically relevant plasma concentrations. Both mouse IMPase 1 (MmIMPase 1) and human IMPase 1 (HsIMPase 1) were cloned into pRSET5a, expressed in Escherichia coli, purified and crystallized using the sitting-drop method. The structures were solved at resolutions of 2.4 and 1.7 angstrom, respectively. Comparison of MmIMPase 1 and HsIMPase 1 revealed a core r.m.s. deviation of 0.516 angstrom.
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Wiley
Subject
Biochemistry and molecular biology, Biophysics
Citation
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Source
Acta Crystallographica Section F, Structural Biology and Crystallization Communications
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Edition
DOI
10.1107/S1744309112035191