Publication:
Cloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase from Mus musculus and Homo sapiens

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dc.contributor.coauthorSingh, Nisha
dc.contributor.coauthorHalliday, Amy C.
dc.contributor.coauthorKnight, Matthew
dc.contributor.coauthorLowe, Edward
dc.contributor.coauthorChurchill, Grant C.
dc.contributor.kuauthorLack, Nathan Alan
dc.contributor.kuprofileFaculty Member
dc.contributor.schoolcollegeinstituteSchool of Medicine
dc.contributor.yokid120842
dc.date.accessioned2024-11-09T11:43:35Z
dc.date.issued2012
dc.description.abstractInositol monophosphatase (IMPase) catalyses the hydrolysis of inositol monophosphate to inositol and is crucial in the phosphatidylinositol (PI) signalling pathway. Lithium, which is the drug of choice for bipolar disorder, inhibits IMPase at therapeutically relevant plasma concentrations. Both mouse IMPase 1 (MmIMPase 1) and human IMPase 1 (HsIMPase 1) were cloned into pRSET5a, expressed in Escherichia coli, purified and crystallized using the sitting-drop method. The structures were solved at resolutions of 2.4 and 1.7 angstrom, respectively. Comparison of MmIMPase 1 and HsIMPase 1 revealed a core r.m.s. deviation of 0.516 angstrom.
dc.description.fulltextYES
dc.description.indexedbyWoS
dc.description.indexedbyScopus
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council
dc.description.versionPublisher version
dc.description.volume68
dc.formatpdf
dc.identifier.doi10.1107/S1744309112035191
dc.identifier.eissn2053-230X
dc.identifier.embargoNO
dc.identifier.filenameinventorynoIR00061
dc.identifier.issn1744-3091
dc.identifier.linkhttps://doi.org/10.1107/S1744309112035191
dc.identifier.quartileQ4
dc.identifier.scopus2-s2.0-84867276609
dc.identifier.urihttps://hdl.handle.net/20.500.14288/346
dc.identifier.wos309357200002
dc.keywordsCrystallography
dc.keywordsInositol monophosphatase
dc.keywordslithium
dc.keywordsbipolar disorder
dc.keywordsMyoinositol monophosphatase
dc.keywordsLithium-theraphy
dc.keywordsPutative target
dc.keywordsBovine brain
dc.keywordsEnzyme
dc.languageEnglish
dc.publisherWiley
dc.relation.grantnoBB/I532929/1
dc.relation.urihttp://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/1093
dc.sourceActa Crystallographica Section F, Structural Biology and Crystallization Communications
dc.subjectBiochemistry and molecular biology
dc.subjectBiophysics
dc.titleCloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase from Mus musculus and Homo sapiens
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.authorid0000-0001-7399-5844
local.contributor.kuauthorLack, Nathan Alan

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