Researcher: Karslıoğlu, Melisa Zişan
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Karslıoğlu, Melisa Zişan
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Publication Metadata only Peripapillary choroidal vasculature in pediatric eyes with type 1 diabetes mellitus(Kare Yayıncılık, 2022) N/A; N/A; Hasanreisoğlu, Murat; Kesim, Cem; Uzunay, Nur Sena; Taş, Ayşe Yıldız; Karslıoğlu, Melisa Zişan; Şahin, Afsun; Faculty Member; Teaching Faculty; Undergraduate Student; Faculty Member; Doctor; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); School of Medicine; School of Medicine; School of Medicine; School of Medicine; N/A; School of Medicine; N/A; N/A; N/A; N/A; Koç University Hospital; N/A; 182001; 387367; N/A; 200905; N/A; 171267Objectives: choroidal vasculature change in children with diabetes mellitus is not investigated enough although it could reflect clinical outcome. Methods: pediatric Type 1 diabetes mellitus (T1DM) patients and healthy controls were retrospectively evaluated. Peripapillary retinal nerve fiber layer optical coherence tomography (OCT) images of the right eyes were analyzed. Choroidal parameters including total choroidal area, luminal area, stromal area, and choroidal vascularity index were measured through image binarization. Results: twenty eyes of 20 patients were compared with 46 eyes of 46 healthy controls. Mean total choroidal area, luminal area, and stromal area were 1.59±0.35, 1.10±0.24, and 0.50±0.13 mm2 in patients’ eyes and 1.52±0.49, 1.05±0.34, and 0.47±0.17 mm2 in healthy eyes. No difference was found in choroidal vascularity indices between patients and healthy eyes (68.8±3.9% vs. 69.4±4.4%, p=0.521). Temporal choroidal vascularity index was significantly higher than its nasal counterpart in healthy eyes (71.8±5.0% vs. 68.6±4.9%, p<0.001) which was not significant in patients’ eyes (70.7±4.0% vs. 68.9±5.1%, p=0.067). Temporal quadrant had the highest choroidal vascularity index score among all quadrants in healthy controls (all p<0.05), whereas no choroidal vascularity index difference between quadrants was detected in patients (p=0.75). Conclusion: peripapillary choroidal vasculature has shown subtle sectoral changes which did not reflect the overall peripapillary OCT section in pediatric T1DM patients when compared with healthy controls.Publication Metadata only Investigation of mitophagy biomarkers in corneal epithelium of keratoconus patients(Taylor and Francis Inc, 2022) Gumus, Koray; Sarac, Ozge Ilhan; Cagil, Nurullah; N/A; N/A; N/A; N/A; N/A; N/A; Yıldız, Erdost; Aydemir, Dilara; Zibandeh, Noushin; Ünlü, Eda Kuşan; Karslıoğlu, Melisa Zişan; Şahin, Afsun; PhD Student; PhD Student; Researcher; Researcher; Doctor; Faculty Member; Graduate School of Health Sciences; Graduate School of Health Sciences; N/A; Graduate School of Health Sciences; N/A; School of Medicine; N/A; N/A; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); N/A; Koç University Hospital; N/A; N/A; N/A; N/A; N/A; N/A; 171267Purpose: The pathological mechanisms of keratoconus (KC) have not been elucidated yet. Mitophagy is an important mechanism that eliminates damaged mitochondria under oxidative stress, and it could be one of the leading pathological causes of KC. This study aimed to find out the role of mitophagy in the keratoconic corneal epithelium. Methods: The corneal epithelia were collected from the 103 progressive KC patients and the 46 control subjects. The real-time quantitative PCR was performed for PTEN-putative kinase-1 (PINK1), PARKIN, p62, and BNIP3 gene expressions in 31 KC and 9 control subjects. Western blot analyses were performed to investigate the protein expressions of PINK1, PARKIN, LC3B, ATG5, and BECLIN in the remaining 109 corneal epithelium samples from 72 patients and 37 control subjects. Results: mRNA and protein expressions of PINK1 decreased significantly in the corneal epithelium of KC patients compared to the control subjects. No significant change was found in mRNA levels of PARKIN, p62, and BNIP3 in KC patients. The protein expression of PARKIN, LC3B, ATG5, and Beclin did not significantly differ between KC patients and control subjects. Gene expression levels of mitophagy biomarkers were not affected by the KC grade. Conclusions: PINK1/PARKIN-dependent mitophagy is affected in the keratoconic corneal epithelium. We found significant decreases in both mRNA and protein expressions of PINK1 in the keratoconic corneal epithelium. However, we did not observe any other significant change in mitophagy markers. Mitochondrial stress-related mitophagy pathways could be interrupted by the decreased levels of PINK1 in the keratoconic corneal epithelium, but solely PINK1 dysregulation is not likely to induce KC pathogenesis.Publication Metadata only Validation results of a deep learning algorithm for detection of diabetic retinopathy with lesion localization from retinal fundus photographs(Assoc Research Vision Ophthalmology Inc, 2020) Ozkaya, Abdullah; Gokgur, Eren; Cakin, Ilgaz; Yavuz, Utku; Baki, Pinar; Chang, Sung-Yen; N/A; Kesim, Cem; Taş, Ayşe Yıldız; Karslıoğlu, Melisa Zişan; Şahin, Afsun; Doctor; Faculty Member; Doctor; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); N/A; School of Medicine; N/A; School of Medicine; Koç University Hospital; N/A; Koç University Hospital; N/A; 387367; 200905; N/A; 171267N/APublication Metadata only The cellular degradative process in epithelial cells of lens capsule with exfoliation syndrome: A preliminary report(Assoc Research Vision Ophthalmology Inc, 2021) N/A; N/A; N/A; N/A; N/A; N/A; Karslıoğlu, Melisa Zişan; Aydemir, Dilara; Sönmez, Sadi Can; Kısakürek, Zeynep Büşra; Şahin, Afsun; Doctor; PhD Student; Other; Undergraduate Student; Faculty Member; Koç University Hospital; Graduate School of Health Sciences; Koç University Hospital; School of Medicine; School of Medicine; N/A; N/A; N/A; N/A; 171267N/APublication Metadata only Choroidal vascularity index in pseudoexfoliative glaucoma(Springer, 2021) Ozum, Yucel; Altan, Çigdem; N/A; Karslıoğlu, Melisa Zişan; Kesim, Cem; Taş, Ayşe Yıldız; Şahin, Afsun; Torun, Şafak; Doctor; Doctor; Faculty Member; Faculty Member; Undergraduate Student; N/A; N/A; School of Medicine; School of Medicine; School of Medicine; Koç University Hospital; Koç University Hospital; N/A; N/A; N/A; 387367; 200905; 171267; N/APurpose To evaluate choroidal vascular involvement in pseudoexfoliative glaucoma (PEXG) by applying the choroidal vascularity index (CVI) to optic coherence tomography (OCT) images. Methods Seventy-eight eyes from 40 subjects were included the study. Group 1 included healthy eyes (n = 20), group 2 eyes with PEX (n = 16), and group 3 eyes with PEXG (n = 42). OCT imaging of macular and peripapillary regions and retinal nerve fiber layer (RNFL) analyses were performed. CVI was calculated using ImageJ software. Results The mean age was 64.89 +/- 5.8, 71.2 +/- 7.8, and 68.24 +/- 7.4 years in groups 1, 2, and 3, respectively (p = 0.046). There were no significant differences between the groups in terms of sex (p = 0.777). In macula, mean CVI rates were 66.97 +/- 1.9%, 64.23 +/- 1.2%, and 64.63 +/- 1.6%, and in the peripapillary areas, mean CVI rates were 67.04 +/- 1.5%, 65.20 +/- 1.5%, and 64.14 +/- 2.1% in groups 1, 2, and 3, respectively (group 1 vs. group 2 and 3, p = 0.000; group 2 vs. group 3, p > 0.05). The decrease in average RNFL thickness was statistically significant in group 3 compared to groups 1 and 2. Conclusion CVI could be used to assess choroidal vascular changes in ocular diseases. CVI was found to be reduced in PEX and PEXG, indicating an ocular vascular involvement in pseudoexfoliative process.Publication Metadata only Does brimonidine tartarate have any effect on viability and/or proliferation of limbal mesenchymal limbal stem cells?(Assoc Research Vision Ophthalmology Inc, 2020) N/A; N/A; N/A; N/A; N/A; N/A; Karslıoğlu, Melisa Zişan; Zibandeh, Noushin; Aydemir, Dilara; Ünlü, Eda Kuşan; Kesim, Cem; Taş, Ayşe Yıldız; Şahin, Afsun; Doctor; Researcher; PhD Student; Researcher; Researcher; Doctor; Faculty Member; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); Koç University Hospital; N/A; Graduate School of Health Sciences; Graduate School of Health Sciences; School of Medicine; School of Medicine; School of Medicine; N/A; N/A; N/A; N/A; 387367; 200905; 171267Purpose : Mesenchymal stem cell transplantation is still one of the hot topics within translational medicine. The limbus, rim of the corneoscleral junction of human eye, is highly rich in terms of stem cells. Limbal mesenchymal stem cells (LMSCs) have been used in many ocular diseases. Brimonidine tartrate (BT) is selective alpha-2 adrenergic agonist that its neuroprotective effect had been proven. BT drops are preferred in glaucoma patients especially for neuroprotection. Our purpose is to evaluate the effect of BT on viability and/or proliferation of LMSCs in vitro. Methods : LMSCs were isolated from corneoscleral rim. The cells in third passage were characterized and differentiated. For MTT assay, cells were seeded in the density of 3x103 in 96 well plate and pretreatment with BT in 0.1, 1, 5,10 and 200 μM concentrations. After 48 hours, MTT solution was added to each wells and plate was read in 570nm absorbance. For scratch assay, cells were seeded in the density of 6x104 in 12 well plate and pretreatment with BT in pervious mentioned concentrations. For Annexin V/PI cell viability test, cells were seeded in the density of 2x105 in 6 well plate and pretreatment with BT in pervious mentioned concentrations. After 48 hours, cells were washed and stained with Annexin V The cells were analysed by flowcytometry. Results : Our experiments showed that pre-treated LMSCs with 10 μM BT increased cell viability significantly compared to untreated cells in MTT assay (p<0.05). In scratch assay pre-treated LMSCs with 5 μM and 10 μM BT displayed statistically significant rapid migration at 20th hours regarding to other untreated cells (p<0.05). According to cell viability results, pre-treated LMSCs with 10 μM BT decreased Annexin V expression compared to untreated group. And it is statistically significant (p<0.05). Conclusions : Since limbal mesenchymal stem cells and optic nerve share the same embryological origin (ectodermal), it is not surp rising that in our study BT enhanced the viability of limbal mesenchymal stem cells and improved proliferation. From this point of view, we hypothesized that intravitreally injection of brimonidine tartrate loaded limbal mesenchymal stem cells may not only protect axon loss, but also induce axon regeneration in traumatic optic nerve injuries. This preliminary study supported our hypothesis and we are in the process of planning future studies about this topic.Publication Metadata only Repeatability of choroidal vascularity index measurements using directional optical coherence tomography images(Lippincott Williams & Wilkins, 2021) N/A; N/A; Ersöz, Mehmet Giray; Kesim, Cem; Karslıoğlu, Melisa Zişan; Taş, Ayşe Yıldız; Hasanreisoğlu, Murat; Şahin, Afsun; Doctor; Teaching Faculty; Doctor; Faculty Member; Faculty Member; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); N/A; School of Medicine; N/A; School of Medicine; School of Medicine; School of Medicine; Koç University Hospital; N/A; Koç University Hospital; N/A; N/A; N/A; 324533; 387367; N/A; 200905; 182001; 171267Purpose: To investigate the repeatability of choroidal vascularity index (CVI) measurements in centered and decentered (regarding pupil entry position of the beam) directional subfoveal optical coherence tomography (OCT) scans with (CVI1) and without (CVI2) brightness adjustment. Methods: Thirty-two eyes of 32 healthy volunteers were included in this prospective study. First, the fovea was evaluated by a single horizontal enhanced depth imaging OCT scan in a centered direction. Then, the same subfoveal horizontal line was scanned in a decentered direction. The agreement between CVIs obtained from these centered and decentered directional OCT scans was investigated using Bland-Altman analysis and intraclass correlation coefficient. Results: Intraclass correlation coefficient between centered and decentered CVI1 was 0.71 (95% CI: 0.48-0.85, P value < 0.001). Intraclass correlation coefficient between centered and decentered CVI2 was 0.73 (95% CI: 0.5-0.86, P value < 0.001). The mean difference between centered and decentered directional measurements for CVI1 and CVI2 were 0.5 and 0.8, respectively. Conclusion: There is moderate agreement between CVIs obtained from centered and decentered directional single OCT scans of the same subfoveal area. Studies investigating choroidal vascularity should be standardized by using the same OCT beam direction in all scans.Publication Metadata only Keratopigmentation: is it a miracle or an adventure?(Kare Yayıncılık, 2020) N/A; N/A; Karslıoğlu, Melisa Zişan; Taş, Ayşe Yıldız; Kesim, Cem; Şahin, Afsun; Müftüoğlu, Orkun; Doctor; Faculty Member; Doctor; Faculty Member; Faculty Member; N/A; School of Medicine; N/A; School of Medicine; School of Medicine; Koç University Hospital; Koç University Hospital; Koç University Hospital; N/A; N/A; N/A; 200905; 387367; 171267; 188588Objectives: this study is an analysis of the cosmetic and functional results of patients who underwent keratopigmentation (KTP). Methods: sixteen eyes of 16 patients, 7 females (43.75%) and 9 males (56.25%) were included in the study. Intrastromal and superficial manual KTP were performed under general anesthesia. Patients with no light perception or with only light perception but total corneal opacification, prosthetic contact lens intolerance, or unwillingness to use a contact lens were studied. The main outcomes were postoperative patient’s satisfaction, cosmetic results, pigment stabilization and surgical complications. A grading system (0-5 points) was used to assess patient satisfaction and the cosmetic results. Results: the mean age of the patients was 30.5±12.06 years (range: 16-53 years). Black pigment was used in 10 patients (62.5%), a brownish color in 5 patients (31.25%) and a greenish, yellow, blue, and black color pigmentation was used for 1 patient (6.25%). The mean follow-up was 29.31±15.45 months (range: 8-52 months). In 2 of 16 patients, mild to moderate pigment loss was seen 12 months after the surgery and superficial KTP was repeated. Minimal pigment loss was seen in 5 patients, but the cosmetic results were satisfactory and no secondary surgical procedure was required. Pigment leakage underneath the conjunctiva was seen in only 1 patient. Otherwise, there were no complications associated with keratopigmentation. The postoperative mean patient satisfaction score was 4.18±0.75 points (range: 3-5 points). Conclusion: KTP is a safe surgical procedure that is easy to learn and perform, does not require expensive materials, and avoids more extensive and invasive reconstructive ocular procedures. Corneal KTP may have a great impact on future ophthalmic surgical practice from both therapeutic and cosmetic perspectives.Publication Metadata only The immunohistochemical analysis of cellular auto-degradative functions in epithelial cells of the anterior capsule of lens in patients with pseudo-exfoliation syndrome: a preliminary report(Assoc Research Vision Ophthalmology Inc, 2022) N/A; N/A; N/A; N/A; N/A; N/A; Sönmez, Sadi Can; Karslıoğlu, Melisa Zişan; Aydemir, Dilara; Gözel, Merve; Kısakürek, Zeynep Büşra; Şahin, Afsun; Undergraduate Student; Doctor; PhD Student; PhD Student; Undergraduate Student; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); School of Medicine; N/A; Graduate School of Health Sciences; Graduate School of Health Sciences; School of Medicine; School of Medicine; N/A; N/A; N/A; N/A; N/A; 171267Purpose : Pseudo-exfoliation syndrome (PXF) is a systemic disease in which fibrillary proteinaceous material is deposited within the tissues such as the eye, which is thought to arise from dysfunctional matrix turnover and intracellular auto-degradation pathways. In this case-control study setting, we aim to evaluate whether defects in mitophagy specifically have a role in PXF etiology and prognosis. Methods : The anterior lens capsules obtained in microincisional cataract surgery were collected both from patients with PXF (n=6) and age-matched healthy controls (n=10). The samples were embedded in paraffin blocks and sliced into 0.4 μm slides for analysis. The ABC® IHC detection kit was used to stain the samples for PTEN-induced kinase 1 (PINK1), Parkin and LC3B. Stained images were captured with 20x magnification under confocal microscopy. The expression intensity was measured using color deconvolution plug-in and integrated density value was calculated on Fiji® (U. S. National Institutes of Health, Bethesda, MD). The Mann-Whitney U test was used for statistical analysis and the results were shown on Graphpad Prism (Graphpad, San Diego, CA). Results : The staining intensity of PINK1 and PARKIN were higher in the epithelia of anterior lens capsule in patients with PXF compared to the control samples, which was only statistically significant for PINK1. (p=0.0002, p=0.999 respectively) LC3B intensity was also found to be increased in PXF patients with statistical significance (p=0.0006). (Images 1 and 2). Conclusions : This increase in the markers of mitophagy, which is the targeted elimination of dysfunctional mitochondria, may be an important indicator of increased oxidative stress in PXF. It can therefore signal an extensive mitochondrial impairment and higher generation of reactive oxygen species, potentially overburdening the cellular clearance and enhancing faulty protein release. Certainly, further in vitro studies with a larger sample number are needed to compare the actual stress responses dynamically in PXF.Publication Metadata only Rhegmatogenous retinal detachments in pediatric population(İnönü Tıp Fakültesi, 2020) Kadayıfcılar, Sibel; Eldem, Bora; N/A; Karslıoğlu, Melisa Zişan; Taş, Ayşe Yıldız; Kesim, Cem; Sevgi, Duriye Damla; Şahin, Afsun; Doctor; Faculty Member; Doctor; Undergraduate Student; Faculty Member; Faculty Member; N/A; School of Medicine; N/A; School of Medicine; School of Medicine; School of Medicine; Koç University Hospital; Koç University Hospital; Koç University Hospital; N/A; N/A; N/A; N/A; N/A; 387367; N/A; 171267; N/AAim: It is to review the clinical features, and surgical, and visual outcomes of rhegmatogenous retinal detachment in a pediatric population. Material and Methods: Medical records of 31 children aged 16 years old or younger with rhegmatogenous retinal detachment were retrospectively analyzed. Cases of acute retinopathy of prematurity were excluded. The data for etiologies, risk factors, prior intraocular surgery, therapeutic approach options, anatomic and functional results were evaluated. Results: Thirty-three eyes of 31 patients with RRD were evaluated. The mean patient age was 11.42±3.82 years (range 3-16). There were 26 (83.9%) males and 5 (16.1%) females. The most common etiology was trauma. At presentation 57.5% of affected eyes (19/33) had a visual acuity worse than 20/400. Preoperative retinal tear was found in 60.6% (20/33) of patients. At initial examination, proliferative vitreoretinopathy was detected in 36.3% (12/33) of patients. The most common primary operation was scleral buckle with subretinal fluid drainage (69.6%, 23/33). Anatomical reattachment was accomplished in 84.8% of cases (28/33). At the final visit, 78.78% of affected eyes (26/33) had a better visual acuity when compared to preoperative examination. In nine of affected eyes (27.27%), visual acuity was 20/200 or better. Mean follow-up time was 37.5 months (range 3-72). Conclusion: In our series, pediatric RRD occurred most commonly in association with trauma. Most eyes were anatomically reattached, and most eyes retained vision of 20/800 or better. Preserving vision in children with RRD is of great importance because of high risk of vision threatening events to the fellow eye.